Title: | Emergence of novel equine arteritis virus (EAV) variants during persistent infection in the stallion : origin of the 2007 French EAV outbreak was linked to an EAV strain present in the semen of a persistently infected carrier stallion (2012) |
Authors: | F. Miszczak ; L. Legrand ; U.B.R. Balasuriya ; Bénédicte Ferry ; J. Zhang ; A. Hans ; G. Fortier ; S. Pronost ; A. Vabret |
Material Type: | Article : printed text |
In : | VIROLOGY (p. 165-174, 2012-01-01) |
Article on page: | p. 165-174 |
Languages: | English |
Descriptors: |
Equivoc Artérite ; Génétique Moléculaire ; Normandie ; Semence ; Stallion ; Virus |
Abstract: | During the summer of 2007, an outbreak of equine viral arteris (EVA) occurred in Normandy (France). After investigation, a link was suggested between an EAV carrier stallion (A) and the index premise of the outbreak. The full-length nucleotide sequence analysis of a study reference strain (F27) isolated from the lung of a foal revealed a 12,710 nucleotides EAV genome with unique molecular hallmarks in the 5'UTR leader sequence and the ORF1a sequence encoding the non-structural protein 2. The evolution of the viral population in the persistently infected Stallion A was then studied bu cloning ORFs 3 and 5 of the EAV genome from four sequential semen samples which were collected between 2000 and 2007. Molecular analysis of the clones confirmed the likely implication of Stallion A in the origin of this outbreak through the yearly emergence of new variants genetically similar to the F27 strain. |
Cote du document numérique : | 15952 |
Classement cheval : | F03 |