Detection of Streptococcus equi subspecies equi us... IFCE

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Titre :	Detection of Streptococcus equi subspecies equi using a triplex qPCR assay (2013)
Auteurs :	K. Webb ; C. Barker ; T. Harrison ; Z. Heather ; K.F. Steward ; C. Robinson ; J.R. Newton ; A. WALLER S.
Type de document :	Article : texte imprimé
Dans :	VETERINARY JOURNAL (THE) (vol.195, 2013-01-01)
Article en page(s) :	p.300-304
Langues:	Anglais
Mots-clés :	Mots-clés cheval GOURME ; MALADIE INFECTIEUSE ; STREPTOCOCCUS Equivoc Bactérie ; Génétique Moléculaire ; Vaccination
Résumé :	Genome sequencing data for Streptococcus equi subspecies equi and zooepidemicus were used to develop a novel diagnostic triplex quantitative PCR (qPCR) assay targeting two genes specific to S. equi (eqbE and SEQ2190) and a unique 100 base pair control DNA sequence (SZIC) inserted into the SZO07770 pseudogene of S. zooepidemicus strain H70. This triplex strangles qPCR assay can provide results within 2 h of sample receipt, has an overall sensitivity of 93.9% and specificity of 96.6% relative to the eqbE singlex assay and detects S. equi at levels below the threshold of the culture assay, even in the presence of contaminating bacteria.
Cote du document numérique :	17122
Classement cheval :	D07/F11/F15

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