|Title:||Detection of anti-GnRH antibodies in horse plasma and steroidomic approach in urine after improvac administrations|
|Authors:||L. Bailly-Chouriberry ; B. Loup, Author ; M.A. Popot, Author ; M.L. Dreau, Author ; P. Garcia, Author ; Jean-François Bruyas, Author ; Y. Bonnaire ; Convention: International Conference of Racing Analysts and Veterinarians ICRAV (20th; 2014; Mauritius), Author|
|Material Type:||printed text|
EquivocAnticorps ; Castration ; Hormone ; Hormone De Reproduction ; Immunocastration ; Vaccination
Since the availability on the European market of the vaccine Improvac dedicated to male pig immune castration, the risk of misuse of this product to horses is now considered as a threat for the horseracing industry. The immunological castration is not allowed by the racing codes (immune system, Article 6). Indeed, this vaccination against the hypothalamic hormone "Luteinising Hormone Releasing Hormone" (LHRH) or "Gonadotropin-Releasing Hormone" (GnRH) will prevent the release from the anterior pituitary of the hormones LH and FSH, which are required for the development and maintenance of the testes in males and ovaries in the female and therefore all their subsequent physiological functions. This treatment will induce a strong hormonal varation resulting in a behaviour modification of the animals.
In this work, we describe a reliable self-made technique based on solid-phase enzyme-linked immunosorbent assay (ELISA) as rapid and efficient screening method applicable to routine analysis. The ELISA test was applied to octanoic acid purified IgGs from plasma samples collected on 4 male standarbreds treated with Improvac vaccine.
The kinetic stydy of plasma anti-GnRH antibodies raised as a consequence of the vaccine administrations will be presented. In order to trace the occurrence of anti-GnRH antibodies in routine analysis and to monitor the animal level exposure to this vaccine, a random population sutdy was conducted on 133 post race/ training horses. Preliminary results obtained on the dimultaneous analysis of urine samples for a steroidomic approach in regards of the sexual status of the animal will be presented.
|Cote du document numérique :||17472|
|Congrès :||INTERNATIONAL CONFERENCE OF RACING ANALYSTS AND VETERINARIANS|