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Quantification of testosterone in the female plasma : a german and french combined study. Contribution the determination of a threshold value

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Titre :	Quantification of testosterone in the female plasma : a german and french combined study. Contribution the determination of a threshold value
Auteurs :	M.A. Popot, Premier auteur ; N. Stojiljkovic, Auteur ; F. Allard, Auteur ; B. Loup, Auteur ; P. Garcia, Auteur ; L. Bailly-Chouriberry, Auteur ; Y. Bonnaire, Auteur ; H. Röttgen, Auteur ; M. Machnik, Auteur ; I. Schenk
Type de document :	texte imprimé
Année de publication :	2014
Format :	p542-546
Langues:	Anglais
Mots-clés :	Mots-clés cheval STEROIDE Equivoc Dopage ; Hormone ; Plasma ; Testostérone
Résumé :	Control of anabolic steroids in plasma in now routinely in place in most of the horse doping control laboratories. Further to the determination of an international threshold value of 100 pg/mL of free testosterone in plasma of geldings, the determination of a threshold value in females is required. In mares, testosterone is principally produced by the ovary and testosterone concentration in plasma varies mainly according to ovarian activity, seasonal and environmental factors. In order to contribute to the determination of at threshold value in plasma, a follow up of eight known mares has been initiated and a population study has been started in France and Germany. From the eight mares engaged in the follow up, variations of testosterone concentrations were recorded form January up to June 2014. As expected individual variations were observed but were in accordance with those oberved in post race sample data. The population studies included 316 post competition samples from Germany and 1007 post reace samples from France. As the two populations were different, statistics were computed seperately. For the French routine population, the mean is 17.2 +- 11.9pg/mL. Using the most appropriate transformations, the obtained tentative thresholds were respectively 99 pg/mL (cube root transformation) and 98 pg/mL (Box-Cow transformation) for a risk of 1/10.000. Regarding the German routine population (n=316), the mean was 10.5 +- 17.4 pg/mL. The data were fitted to a Gamma distribution and a tentative threshold value of 113 pg/mL. was calculated for a risk of 1/10.000. Keeping in mind that 100 pg/mL is the threshold value in place for gelsings, a higher threshold value is expected for testosterone in plasma of mares. This needs to be checked with additional data from other countries. The foilow-up study of the eigth mares is still ongoing and ill be finished early 2015.
Cote du document numérique :	17475
Classement cheval :	F11
Congrès :	INTERNATIONAL CONFERENCE OF RACING ANALYSTS AND VETERINARIANS

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